Sentence examples for binding was performed for from inspiring English sources

MMP2 aptamer or anti-MMP2 antibody (AB37150, Abcam, Cambridge, England, UK) binding was performed at a dilution of 1 200 in blocking buffer overnight at 4°C, and secondary antibody (horseradish peroxidase-conjugated anti-rabbit, 1 5,000) binding was performed for 2 h at RT.

Subsequently displacement binding was performed for each ligand in the presence and absence of a constant concentration of T30 (100 nM).

Blocking against non-specific binding was performed for 60 min with 0.5 % goat serum dissolved in PBST, and the cells were again washed three times with PBST.

Blocking against non-specific binding was performed for 1 h in 5% normal donkey serum for b-tubulin III or bovine serum albumin (BSA) for F-actin and vinculin staining.

Binding was performed for 2 h at 37°C.

Binding was performed for 1.5 hours at room temperature followed by three rapid rinses in PBS/0.05% Tween-20.

Triplicate binding were performed for each donor and concentration of ligand.

After washing for three times, cell lysate was mixed with the resin, and binding was performed at 4°C for 2 h.

Anti-FLAG M2 affinity gel (30  μl; Sigma) was mixed with lysate from cells transfected with FLAG-tagged protein, and binding was performed at 4°C for 2 h.

G-CSF binding was performed by incubating splenocytes for 1 h with 25 ng of mG-CSF (ProSpec, East Brunswick, NJ, USA) biotinylated with a Biotin XX Microscale protein labeling kit (Molecular Probes, Eugene, OR, USA), detected by flow cytometry with PE-streptavidin.

The incomplete overlap of TAF9B and OLIG2 peaks may in part represent intrinsic differences between progenitor cells and post-mitotic neurons, since the OLIG2 binding was performed with neuralized EBs enriched for progenitor cells while TAF9B mapping was done with EBs enriched for post-mitotic motor neurons.