Sentence examples for binding was performed by from inspiring English sources

Blocking of non-specific antibody binding was performed by incubation with normal goat serum at 37 °C.

Blocking for non-specific binding was performed by adding 100 μL of 3% bovine serum albumin (BSA) and incubating for 60 min at room temperature, followed by three PBS washes.

Confirmation of the binding was performed by PCR using mature miR-199a* as a primer and the targeting was performed by luciferase assays.

Quantification of Annexin V-FITC and PI binding was performed by a FACScan (BD Biosciences) using channels FL-1 (Annexin V-FITC) and FL-3 (PI).

Measurements of the formation of Mcm binding was performed by introducing the mcm6 GFP allele [6], [11] and to visualise the presence of the tagged protein in Triton-extracted cells by fluorescence microscopy.

Detection of antibody binding was performed by using the following secondary reagents: DAKO peroxidase EnVision system for the detection of mouse and rabbit antibodies, and Sigma biotinylated anti-goat antibody (followed by extra avidin peroxidase reagent) for goat antibodies.

[H]Paroxetine binding was performed as described by Marazziti and colleagues [ 25].

The tissue binding was performed over different HA concentrations by diluting the pre-complexed HA in 1% BSA-PBS.

Filter binding was performed using nitrocellulose filters followed by three washes with 5% (w/v) chilled TCA, and radioactivity was determined.

PAMPs binding assay was performed by the method of PAMPs microarrays, and the binding activity was calculated by the signal value of fluorescence.

Inspection of this sequence for the presence of consensus transcription factor binding sites was performed by high stringency searches using PATCH (Pattern search for transcription factor binding sites) and TRANSFAC 6.0.