Sentence examples for binding was performed at from inspiring English sources
The major part of reported binding was performed at neutral pH as β-lg does not bind easily hydrophobic ligands at low pH.
Elution yields were consistently higher when EPO binding was performed at low ionic strength.
MMP2 aptamer or anti-MMP2 antibody (AB37150, Abcam, Cambridge, England, UK) binding was performed at a dilution of 1 200 in blocking buffer overnight at 4°C, and secondary antibody (horseradish peroxidase-conjugated anti-rabbit, 1 5,000) binding was performed for 2 h at RT.
The OMAB antibody was immobilized at a density of 10 000–15 000 RU to a CM5 chip (GE Healthcare) using standard amine-coupling chemistry at pH 6. Analysis of Aβ binding was performed at a flow rate of 50 µl/min in PBS at 25°C.
After washing for three times, cell lysate was mixed with the resin, and binding was performed at 4°C for 2 h.
To measured binding affinity, competition binding was performed with 0.6 nM [H]granisetron (close to Kd for this ligand) for 120 min. In these experiments, binding was performed at 19°C to inhibit receptor endocytosis.
The experiments with TBP/ODN binding were performed at 25°C in binding buffer (20 mM 4-[2-hydroxyethyl]-1-piperazineethanesulfonic acid [HEPES]-KOH pH 7.6, 5 mM MgCl2, 70 mM KCl, 1 mM dithiothreitol [DTT], 100 μg/mL BSA, 0.01% NP-40, and 5% glycerol) at a fixed concentration (0.3 nM) of active TBP.
Karytyping and G-binding was performed as previously described [36] at Chinese Academy of Medical Science & Peking Union Medical College.
Blocking of non-specific antibody binding was performed by incubation with normal goat serum at 37 °C.
Blocking for endogen peroxidase and unspecific binding was performed.
