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Briefly, binding was optimal for lipid mixtures with an overall negative charge of 50% [24] achieved by 1∶1 mixtures containing either DOPG or PBPS as the negative component, and EYPC as the zwitterionic component.
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In E.coli, it has been demonstrated that the 6 bp-distance between the XerC and XerD binding sites was optimal for chromosomal recombination activity and cleavage [52], [53].
With CH58, a network of structural changes involving such mutations acted to configure the conformation of LCDR3 from one that would only tolerate antigen binding to one that was optimal for it.
Using a synthetic TZF domain peptide derived from the human TTP, known as TTP73, our group independently showed that this same nonamer sequence was optimal for TTP binding on gel-shift analysis, which could be accomplished with binding affinities in the low nanomolar range [ 31].
HLA did not bind to any of the LUVs under physiological conditions (Fig. 1A), confirming previous studies where a pH<5 was optimal for HLA binding to bilayers with 50% negatively charged phospholipids.
Furthermore, a "Goldilocks zone" must exist for each protein where the actin-binding affinity must be optimal for accumulation.
The ELISA binding assay for 89Zr-bevacizumab gave a binding of 75%, which is optimal for this assay, and did not alter upon coupling of 1 of 2 eq of dye.
Derivatization on substituted phenyl groups at the 5- and 7-positions of 2 revealed that a 3,4-methylenedioxyphenyl group at the 5-position and a 4-methoxyphenyl group at the 7-position were optimal for binding affinity.
Examination of the docking results revealed that TP5 was predicted to be optimal for binding into the cleft within the MHC molecules.
Highly sulfated forms can fail to support adhesion, whereas low-sulfated forms are optimal for binding [69], [70] and appear on the syncytiotrophoblast and in intervillous spaces [70].
The T and C in this position are optimal for binding the B-ZIP proteins CREB and C/EBP, respectively (Johnson 1993).
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