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Exact(40)
The GST-p53(295–390) mutant keeps the ability to bind PATZ1, whereas no binding was observed with the GST-p53(13–295).
Using GST or GST-NEMO as bait proteins (Figure 3A), we found that each of the five interactors was able to specifically bind GST-NEMO, while little or no binding was observed with the GST control (Figure 3B).
Highest cooperative binding was observed with DNA having maximum T-T mispairs which was supplemented by detailed UV-visible thermal analysis of DNA-Hg II) structures.
Evidence of disulfide bond formation was observed with the [B20H17SH]4− anion, whereas no evidence of covalent binding was observed with the [B20H18]2− and [B20H17OH]4− ions.
It bound specifically to the constant region of the rabbit IgG, and no binding was observed with mouse or goat IgG.
No binding was observed with biotinylated-BSA alone (Fig 1C), indicating specific binding to the INr peptides.
Similar(20)
The nucleophilic water molecule is displaced and a unique mode of binding is observed with the S-OH of the inhibitor occupying the oxyanion binding site of the proposed tetrahedral intermediate.
If a similar oligonucleotide junction is used that does not contain ICRIII (Junction 2 in Figure 2), much weaker binding is observed with a Kapp of 379 nM (inset Figure 3a).
As for exon10 SH3 domain binding, ionic strength-sensitive PRD SH3 domain binding was observed, albeit with lower binding affinity.
Higher binding was observed compared with that seen in APP/PS1 mice, which expresses the Swedish APP and presinilin-1 transgene (Alzheimer-like amyloid pathology model).
Furthermore, high affinity SrbA161-267 binding was observed as well with putative binding sites that had only one mismatch compared to known binging sites in S. pombe.
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