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Exact(18)
However, only a small increase in binding was observed when the Hb concentration increased.
A marked decrease in ATF4 binding was observed when mice are refed 2 h with a control diet after leucine deprivation.
Fig. 2D shows that GST-PBD bound to NPM/B23 while no binding was observed when GST-alone was incubated with NPM/B23.
In addition, both scFvs, moScFv and huScFv, recognized the peptide P1, whereas no binding was observed when peptide P1Q (prolongedged for a single Glu residue at the C-terminus) was used as an antigen in ELISA (Figure 4).
Dose-dependent and saturable binding was observed when increasing concentrations (0 to 1,000 nM) of the recombinant proteins MPL36, LipL40, LipL32, rLIC10494 and rLIC12238 were allowed to individually adhere to a fixed PLG amount (1 µg).
No binding was observed when only the N-terminal 35 amino acids of Tat were included in the reaction, indicating that these residues are not involved in RSK2 binding (Figure 3D, lane 2).
Similar(42)
Consistent with this, substitution of Phe54 at the tip of the HCDR2 loop of CR6261 with leucine or alanine reduced binding in relation to hydrophobicity, and additive decreases in binding were observed when CR6261 mutants and HA mutants were combined (Fig. 9b).
No discernable binding is observed when the drug is titrated against the mitochondrial extract from B2 cells lacking in complex-I.
No inhibition in STAT1 DNA-binding was observed when COS-7 nuclear extracts containing IFN- γ-induced activated STAT1 were incubated in the presence of 30 μM of compound and S3I-201 (Supplementary Figure S2).
Moreover, 2H-5 binds weakly to competitor tRNA as no binding saturation was observed when up to 2 μM tRNA (Kd ≫ 2 μM) was added (4-fold excess over 2H-5), in agreement with IC50 values in the presence and absence of competitor tRNA, which do not change (Table 1).
The best binding activity was observed when the ratio of RelB/RelE reached 2∶1 (Fig. 2B, lane 6).
More suggestions(15)
binding was enhanced when
binding was regained when
binding was abolished when
binding was abrogated when
binding was noted when
binding was retained when
binding was restored when
binding was detected when
binding were observed when
binding was found when
binding was inhibited when
binding was achieved when
binding was seen when
binding was lost when
binding was evident when
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