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EMSA experiments demonstrated that CRTR-1-containing complexes could specifically bind DNA containing a CP2-response element (Figure 4A), as no competition for binding was observed using an oligonucleotide containing a mutated CP2-response element.
However, no binding was observed using the COOHV chip, a 3D Dextran chip.
No apparent loss of binding was observed using the conjugates of MAb 17.1A on Colo 320 cells.
The SbMYB44 protein showed increased binding with increasing concentration of the probe, the maximum binding was observed using 80 ng probe with all the cis-elements (Fig. 5A and B).
Similar(56)
Similar trends in Kef binding were observed using DSF.
As expected, both anti-CD9 and anti-CD41b demonstrated significant signals for binding using CCS compared to that from CCS filtrate, and nearly no binding signal was observed using cell culture medium, indicating that exosomes in CCS were captured by these two antibodies.
A concentration-dependent binding displacement was observed using human tissue samples for both cold competitors.
Little activation of 3-EA to DNA-binding metabolites was observed using S9 from three of the four species, mouse being the exception.
Lastly, decreased binding of NFκB was observed using luciferase reporter assay in NFκB knock down cells.
The 3′ region of the intron also possessed putative Sp1 binding sites (oligo-6); however, no transcription factor binding to this sequence was observed using the same method (data not shown).
Given these variations in targeting profiles and binding affinity, slightly lower SNR was observed using microPET imaging.
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