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c When 99mTc-SER-4 was added to Sn-Fc protein in the presence of unlabelled SER-4, no binding was observed and only the 99mTc-SER-4 elution peak was observed.
Robust binding was observed and different regions displayed variable binding efficiencies.
The response of the GUV to daptomycin binding was observed and recorded by a camera-equipped microscope.
In this single example of target co-regulation by Tsh and Scr the binding sites of the respective transcription factors are not immediately adjacent and no cooperative binding was observed, and therefore it has been cautioned that without additional data Tsh should be considered a genetic collaborator rather than a cofactor (Mann et al., 2009).
Binding was observed and was dependent on the presence of Ran-GTP (Fig. 1A and B).
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Above 100 mM K+, only specific binding is observed, and both the binding constant and the magnitudes of enthalpy, entropy and heat capacity changes all decrease strongly with increasing [K+].
Conversely, GLP-1 receptor mRNA is concentrated in the PVN where only weak binding is observed and where ir-GLP-1 fiber terminals are closely associated with OT -and CRF-expressing neurons [324].
Approximately 50% loss in binding was observed with 5 and 1 μg/ml elastase treatments of ECM and fibronectin respectively, while high doses of elastase (50 μg/ml) resulted in >90% loss of binding to either matrix.
In contrast, a loss of binding was observed for ZO1 PDZ2 and the PDZ domain of SHANK1 when the histidine and valine residues were mutated to leucine and lysine or serine and glutamic acid, respectively (Fig. 5C D).
Surprisingly, however, no detectable binding was observed between NBMPR and hENT1 by ITC (data not shown).
However no binding was observed between CD40 and C4BP under any conditions.
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