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a Clear tracer binding was observed after incubating pancreatic tissue sections with [18F]exendin-4 (2.5 nM) b co-localization with the islets was observed by staining the same sections with insulin antibody.
An enhancement of DBS binding was observed after addition of NTD125 (0.5, 1.0, 1.5, and 2.0 µg) in the reaction mixture (Fig. 5a, lanes 4, 5, 6 & 7).
Only External binding was observed after incubation at 4 °C, excluding unspecific internalization.
Complete inhibition of binding was observed after pretreatment of the recombinant VLPs with type A secretor saliva, and binding was markedly reduced after incubation with type O secretor saliva.
In smooth muscle DDT1 MF-2 cells, uncoupling of A1 receptors from G proteins (measured by a decrease in agonist binding) was observed after 30 min exposure to agonist, an effect involving receptor phosphorylation and arrestin binding [ 24].
Binding was observed after 90 120 min and increased gradually suggesting that aptamer binding correlated with the increase in β-sheet content.
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It is evident after one minute incubation, and maximum binding is observed after 15 min (Fig. 1).
A concomitant increase in c-Jun DNA-binding was observed after 6 h stearate treatment (2-fold, Figure 2F).
In DNA-binding assays we observed a trend towards elevated p50 and p65 binding 1 h after IL-1β stimulation in the presence of TSA, while a significant reduction of DNA binding was observed only after 24 h (figure 5C).
In their native locations, the peak of Cdc45 binding was observed at 30 min after G1 release on ARS605, at 40 min on ARS409, at 50 min on ARS501 and at 70 min on ARS609.
The adsorption on parylene-C showed almost no reversibility (1% mass difference after 5 min rinsing) whereas more reversible binding was observed on SiON (10% mass difference after 5 min rinsing) (Table S2).
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