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Direct binding was not demonstrated.
However, significantly higher binding was not demonstrated within CA NT tumours after exposure of tumour-bearing animals to hypoxic conditions.
Others have not confirmed evidence of DNA adducts (or of an OTA-dGMP adduct) in rats following OTA exposure using P-post-labelling (Mally et al. 2004, 2005b) and DNA binding was not demonstrated in a few studies employing H- or C-OTA (Schlatter et al. 1996; Gautier et al. 2001; Gross-Steinmeyer et al. 2002; Mally et al. 2004).
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Some of these proteins are known spindle components, however their direct binding to microtubules was not demonstrated before.
Impaired production of inhibitors of the Jak-STAT pactivatedctivated by interferon binding to IFNAR was not demonstrated by the authors, as SOCS1, SOCS2 and PIAS1 expression was comparable between responders and nonresponders.
Immunofluorescence staining of MCF7 cells transfected with the S100A14-GFP expression vector using S100A16 antibody showed colocalization of S100A14 and S100A16 proteins on the cell membrane of MCF7 cells, although the binding of these proteins to each other was not demonstrated in the pull-down assay (data not shown).
Stability was not demonstrated.
Functional studies have suggested interactions with the nuclear export protein CRM1 [ 50], although direct binding interactions were not demonstrated in that study.
First, we showed that SP-A binding was not inhibited by EDTA, demonstrating that the SP-A carbohydrate recognition domain was not required.
We also performed ChIP assays in cells derived from Runx1−/− ES cells to demonstrate that factor binding was not an artifact of leakiness of the inducible system.
Since our data demonstrate that NEUROD2 binding was not exclusive to promoter regions or TSS-proximal sequences, we decided to employ a method for assigning individual peaks to genes which would also account for TSS-distant peaks, as opposed to methods which set fixed windows around TSSs for peak capturing.
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