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For each brain, specific binding was normalized to the average specific binding from brains in the vehicle-treated group.
Fold enrichment of E2f1 binding was normalized to rabbit IgG.
After grafting, F-fallypride binding was normalized to intact levels as assessed 5 months posttransplantation, indicative of active DA release from the transplanted hESC-DA neurons.
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To account for slight differences in assay efficiency (hybridization, purification, and binding) the data was normalized to the sum of 6 positive RNA spike-in controls.
Binding of mutant proteins was normalized to binding of MBP-P66M to allow incorporation of data from multiple experiments into the statistical analyses and figures, as raw optical density (OD) values were not always identical in different experiments.
For each gene, enrichment of TBP or POL2 binding to the promoter was normalized to a no antibody control and for non-specific recruitment at a control locus.
Binding of bacteria expressing mutant P66 proteins was normalized to binding of WT bacteria to allow incorporation of data from multiple experiments into the statistical analyses and figures, as raw OD values were not always identical in different experiments.
Pou3f1 enrichment at identified binding sites of each gene was normalized to corresponding coding regions.
Specifically, when the protein binding of the "wild-type" allele was normalized to 100%, rs6772676-T allele showed lower protein binding (only 28%) compared to the rs6772676-C allele.
NRSF occupancy at NRSE sites was normalized to IgG binding to the DNA and calculated as a percentage of total input.
Each sample was assayed in quadruplicate and expression of p62/SQSTM1 was normalized to the Tata binding protein (Tbp).
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