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The exact binding strength at 250 mM and 500 mM NaCl may be inaccurate, due to the fact that binding was much weaker and saturation could not be achieved in this assay.
Some Monkey King fragments (ES6, ES11, and ES12) were also recognized by nuclear proteins from Tapidor leaves; however, the binding was much weaker than to the ES7 fragment.
Immune sera also bound to neu protein on the surface of NT2.5 tumor cells at a 1 100 dilution, although the binding was much weaker (approximately 10-fold) than binding to HER2 protein.
For the second variant R1699W, binding was much weaker, and the Kd could not be precisely determined (>200 μM), corresponding to at least a 160-fold reduction in affinity compared to wild type.
Drp1 and hFis1 were shown to coprecipitate in IP experiments, in keeping with a previous report (Yu et al, 2005), but the hFis1 Drp1 binding was much weaker than that between hFis1 and MIEF1.
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In accordance to our data, they found that this binding is much weaker in the presence of serum.
This particular order of binding reactions was chosen for the formation of the trimeric complex since previous measurements have shown that FGF2-FGFR binding is much weaker than the binding of FGF2 to HSGAG.
Intriguingly, NADH binding is specific in the sense that NAD+ binding is much weaker (Kd > 250 μM), presumably due to the presence of two positively charged arginine residues (Arg143 and Arg219) near the nicotinamide ring of NADH (see Figure S1).
For example, in some instances the TF binding is much weaker than expected, in other cases this association is complicated by the occurrence of divergons, which are pairs of divergently transcribed operons or genes, or by the presence of binding sites within coding regions.
In acetonitrile complete release of the template could be observed within 15 min. We have to point out, however, that the template binding is much weaker in acetonitrile compared to toluene which is attributable to the polar nature of this solvent.
In contrast, HKII binding to mitochondria was much weaker and readily displaced by excess HKI.
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