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24b VLP binding was monitored at 22 °C after inducing domain growth by heating the chamber to 50 °C (one hour) and cooling it down to 22 °C, in analogy to the AFM experiments.
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The binding was monitored during one minute injections at 50 μl/min at concentrations ranging from 10 nM to 50 μM.
Binding was monitored by the colorimetric conversion of nitrocerfin by a β-lactamase fusion to DCC.
The protein binding was monitored by FET and direct current (dc) measurements.
Nonspecific binding was monitored using IgM as control sample.
PAPS binding was monitored via binding-induced changes in the intrinsic fluorescence of SULT1A1.
Metal binding is monitored by the appearance of a new LMCT band at 238 nm.
was monitored at 450 nm to detect binding of human TIM proteins with Ig V domains to PS liposomes immobilized on plastic plates (See MATERIALS AND METHODS).
Steady-state equilibrium binding of conformation dependent human H5N1 neutralizing MAb FLA5.10 was monitored at 25°C using a ProteOn surface plasmon resonance biosensor (XPR36, BioRad Labs).
The progress of apoptosis was monitored at the same time by Annexin-V binding assay.
However, when XAC-X-Texas Red (15) was monitored at the single-cell level, no specific binding was seen at concentrations up to 30 nM.
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