Sentence examples for binding was minimized by from inspiring English sources
Non-specific Ab binding was minimized by incubating the plates with 4% blocking solution (Block Ace; Dainippon Sumitomo Pharmaceuticals, Osaka, Japan) at 37°C for 2 h.
Briefly, non-specific antibody binding was minimized by incubating sections for 90 min in diluted normal blocking serum.
Nonspecific binding was minimized by using protein block (Dako).
Non-specific protein binding was minimized by pre-treatment with 20% normal swine serum (Dako, Glostrup, Denmark) and endogenous peroxidase was quenched with 0.3% hydrogen peroxide.
For NF-κB p65 staining, cells were fixed in acetone and ethanol (1 1) for 20 min, and then non-specific binding was minimized by blocking with 2% BSA.
Excess background and non-specific binding was minimized by washing the filters at 60°C with a series of buffers (2x SSC, 2x SSC with 0.1% SDS, 1x SSC with 0.1% SDS, and 0.5x SSC with 0.1% SDS).
Endogenous peroxidase activity was blocked by 3%H2O22, and nonspecific binding of immunoglobulin was minimized by blocking with Super Block (Scytek Laboratories, Logan, UT, USA) for 1 h at RT.
Non-specific binding of antibody reagents was minimized by incubating each slide for 30 min at RT with serum from the same species from which the fluorochrome-conjugated antibodies were derived.
When chimeras were diluted significantly (e.g., dose response experiments in Figures 3– 5), binding of protein to tubes was minimized by the presence of 1% BSA in diluent (final 0.01 to 0.03% BSA).
Non-specific binding on the nitrocellulose filter paper was minimized by blocking for 1 h at RT with TBS-T buffer [25 mM Tris-HCl (pH 7.5), 150 mM NaCl, 0.05% Tween 20] containing 5% (w/v) nonfat dry milk.
