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Similar binding was measured with additional two human MAbs (data not shown).
Glycine betaine binding was measured with three different methods: (i) intrinsic protein fluorescence; (ii) isothermal titration calorimetry; and (iii) a filter-based assay using radioactive ligand.
Ligand binding was measured with short CLE-like peptides.
ATP binding was measured with Rho-MatB in large excess over the nucleotide.
The level of binding was measured with marbofloxacin concentrations of 0.05, 0.5 and 5 μg/mL.
However, after 24 h of hypoxia, MBP-1 binding to the P2 promoter drastically decreased and the greatest reduction in binding was measured with 25 mM glucose.
Similar(54)
The kinetics of XMP binding were measured with an Applied Photophysics SX.17MV stopped-flow spectrophotometer by monitoring the changes in protein fluorescence (excitation = 295 nm, 320 nm cutoff filter).
Increased [18F]-PBR111 binding was measured in vivo with PET which corresponds with the brain regions with increased brain inflammation identified with [125I]-CLINDE autoradiography and OX-42 immunohistochemistry (Figure 5).
Nucleoside diphosphate (NDP) substrate binding was measured by competition with a fluorescent derivative of ADP, following the fluorescence anisotropy of the derivative.
For each antibody population, the specificity of the binding was measured by incubation with 100-fold excess of unlabeled GAD in homologous and heterologous inhibition assays, and the affinity of binding with GAD65 and GAD67 was measured in selected sera.
In more recent PET studies, low C-PK1195 binding was measured in patients with Alzheimer's disease and mild cognitive impairment (Okello et al., 2009; Wiley et al., 2009).
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