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Expression/Ab binding was measured using flow cytometry.
In all experiments Ab binding was measured using flow cytometry and respective isotype controls were subtracted.
Nucleotide binding was measured using a standard filter-binding assay.
Microtubule binding was measured using the Microtubule Protein Spin-Down Biochem Assay Kit (Cytoskeleton, Inc).
A different kinetic and quantitative assessment of peptide binding was conducted using another flow cytometry assay in which binding was measured using an anti-biotin mouse monoclonal antibody conjugated to AlexaFluor 488 (Invitrogen).
Ligand binding was measured using FITC-labeled TGF-β1 and flow cytometric analysis.
Similar(43)
Total binding and non-specific binding were measured using a quartz crystal microbalance with dissipation (QCM-D).
Assembly of the array, attachment of antibodies and antigen binding were measured using surface plasmon resonance and neutron reflection.
Nuclear extracts from the cells were prepared and p65 DNA binding activity was measured using the TransAM™ NFκB kit according to the manufacturer's instructions (Active Motif, Carlsbad, CA).
The size of the binding pocket was measured using the Docking Simulation Module in Sybyl.
The binding strength was measured using a nano Isothermal Titration Calorimeter (ITC TATA instruments, Newcastle, UK).
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