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ATP binding was measured at 10 °C to avoid hydrolysis of the nucleotide.
To explore this discrepancy further, DNA binding was measured at lower concentrations of alkylanilines using human S9.
This is a significantly higher free fraction than the estimates for free fraction in the brain, where protein binding was measured at approximately 99.9%.
The immunolabeling assays were performed as previously described and the degree of antibody binding was measured at 620 nm after a reaction time of 40 min to enable color development.
Binding was measured at 25°C by surface plasmon resonance.
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Antibody binding was measured as described above.
Briefly, the extent of TM-BBB4 cell-[I]hAβ 1-40) (0.15 μlin200 μl in ECF buffer) binding was measured for 3 min at 37°C.
The ANS (8-anilino-1-naphthalene-sulfonate) binding was measured by fluorescence emission with excitation at 380 nm and emission was recorded from 400 to 600 nm.
The aptamers were incubated with the target cell line at 37°C and 4°C and their binding was measured.
DNA binding was measured using FRET and employed 21 base pair duplexes labelled at their 5′ ends with hexachlorofluorescein (HEX).
Nucleotide binding was measured using a standard filter-binding assay.
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