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The percent fluorescence quenching of uGRPs labeled with MDCCin solution containing 10 3 M glucose is shown in Supporting Information, Table 3. Glucose binding was maintained with the GRPs containing unnatural amino acids.
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It is important to note that although PIP2 binding was maintained, the loss of interaction with the 4-phosphate moiety of PIP2 results in loss of cooperative binding and therefore ablation of oligomerization, which is critically dependent on R40 forming 'bridging' interactions between two neighboring PIP2 molecules.
We also show that A8-35 solubilized MPs are transferred into the gas phase with lipid binding being maintained, thus permitting the study of MP/lipid interactions by ESI-IMS-MS, which is fundamentally important for attaining mechanistic insights into MP function.
Moreover, the CEU original covalent binding by an ester linkage on β-tubulin Glu198 and prohibitin Asp40 was maintained with Oxas.
Anesthesia was maintained with isoflurane.
Anesthesia was maintained with sevoflurane.
Although wt bZIP still targets AP-1 with strongest affinity, both DNA-binding specificity and affinity are maintained with wt bZIP binding to noncognate gene regulatory sequences: the dissociation constant for wt bZIP in complex with AP-1 is 13 nM, while that for C/EBP is 120 nM, XRE1 240 nM, and E-box and HRE are in the μM range.
After incubation at 37 degrees C for 4 days, the binding affinity was maintained at 60% of initial levels.
Binding activity of antibodies was maintained, and in vitro binding of target antigens was observed.
In contrast, it was maintained for Np647 ExCys1 (Fig. 4a), consistent with the binding of the probe to the GLP-1r of beta cells.
Binding of a single dimer to S1 was maintained when S2 was disrupted, whereas disruption of S1 eliminated any significant binding to S2, also consistent with positive cooperativity.
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