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Fibronectin binding was localized to IG4-5 and fibulin-2 binds to IG2 and IG13-15 with different affinities.
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Together, these data indicate that while multiple domains of Smad3 are capable of interacting with Sox17, the strongest binding is localized to the linker and MH2 regions from amino acids 146 425.
In parallel with our in vitro studies, a punctate distribution of FXa was observed, in accord with the notion that FXa binding is localized to the platelet surface during thrombus formation.
The results demonstrate that ssDNA binding is localized to the C-terminal region that contains the OB-fold domain, while a tandem helix-hairpin-helix (HhH 2 motif contributes significantly to dsDNA binding.
In other figures, it was critical for us to be able to test just the motor domain to show that Rab6 regulation of KIF1C's MT binding is localized to the motor domain alone.
However, in an in vitro binding assay in which regions of recombinant PTPμ were expressed [ 17], the homophilic binding site was localized to the immunoglobulin (Ig -like domaIg -like
The RyR2 binding site was localized to a highly conserved 150-amino acid residue region.
These results agreed with the finding that the binding site for β1 was localized to DIV in rNav1.2 [33] and implicated that TipE might not regulate DmNav1 through DIV.
The collagen binding site of α2 β1 integrin was localized to the α2 von Willebrand factor type A (vWFA) domain [ 28].
According to the crystal structure of DNA bound EGR1/Zif268, the residues most crucial for DNA binding are localized at positions -1, 3 and 6 with respect to the start of the α-helix.
Antibody binding was localized with appropriate AlexaFluor-conjugated secondary antibodies (Invitrogen, Carlsbad, CA).
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