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The specificity of NF-κB binding was indicated by competition with unlabelled probe and an unrelated competitor (activator protein-1 oligonucleotide; data not shown).
The ability to express curli fimbriae was evaluated by streaking each strain on modified LB-agar plates (without NaCl) containing 0.004% Congo Red (CR) and 0.002% Coomassie Brilliant Blue G. CR binding was indicated by the presence of red or pink colonies after incubation overnight at 37°C.
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The binding pockets for HT ligand binding are indicated by orange arrows.
The residues participating in site 1 binding are indicated by triangles.
The completely conserved residues indispensable for DNA-binding are indicated by middle dot.
The sTfR binding activity was indicated by OD values.
The binding event was indicated by a decrease of a signal from the DNA label.
Irreversible binding, however, was indicated by no changes in DH after four filtrations with strongly bound catechol PEG conjugates.
The binding specificity was indicated by the controls using the GAPDH promoter and non-specific IgG and the lack of detection of the ΔNp63α-GLI2 promoter complex in SaOS-2-EV cells.
Additional ligand-binding potential was indicated by the Phyre 2.0 3DLigandservererver (http://www.sbg.bio.ic.ac.uk/3dligandsite/) for which confidence values had an average LnE ≥10 for all PAS domains (average LnE range of 9.0-13.4, with a value of >4.0 considered significant) (Wass et al. 2010).
(A ) Screening schematic: chemicals of diverse structure were covalently attached to a functionalized glass surface, and incubated with protein of interest, the binding of which was indicated by primary antibodies recognized by a specific fluorescently (Cy5, red) labeled secondary body.
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