An additional negative regulatory region of p53 sequence-specific DNA binding was identified in proline rich region spanning aa residues 80 93, furthermore, synthetic peptides from this region (aa 80 93) are able to activate p53 DNA binding activity in vitro [11].
Using a Taq polymerase stop assay to identify the site of DNA adducts, a novel pattern of DNA binding was identified in pBR322 DNA after incubation with 10 and 100 μ M AMD473 for 2 h (Holford et al, 1998a).
A total of 877 transcription factors (TF) distributed into 30 families on the basis of conserved structural domains involved in DNA binding were identified in the maritime pine transcriptome [ 10].
Moreover, four other conserved amino acids, Asp-338, His-343, Arg-406 and His-528, likely involved in substrate binding, were identified in RHA1 GlgB, based on the amino acid sequence alignment.
Preferential AFB1 bindings were identified in both PLC and hHC DNAs compared to normal liver DNA when analyzed by restriction endonuclease digestions and agarose gel electrophoresis.
A putative consensus NtcA binding site was identified in binding regions #204, 364, 602, 892, 996, 1128, 1137, 1203 and 1570, while binding regions #602, 996, 1128 and 1203 were found close to a TSP (Class II-dependent acompatiblecompatible sites).
For example, WRKY71 for whom a binding site was identified in the OsEULS2 promoter was upregulated upon infection with Magnaporthe grisea (Berri et al. 2009).
A potential CRP binding site was identified in the promoter region of ler.
The diphosphate binding site was identified in the putative active site of both models in close proximity to N102.
The conserved location of transcription factor binding sites was identified in mammalian alignments.
A protein, VC1059, annotated as a short chain dehydrogenase containing an NADPH binding domain, was identified in the active fraction.
