Sentence examples for binding was generated by from inspiring English sources

Our multi-recruiter model of Tup1 binding was generated by regression analysis.

The p851ΔEbox reporter construct containing a CA→TC mutation to prevent Ebox protein/DNA binding was generated by PCR.

miR-9-2 promutantmutant for Hes1 binding was generated by deleting the following nucleotides (underlined): Nbox2 – aC ACTAGcgc; Nbox3 – taG TGCTAgtg).

miR-9-2 promutantmutant for Hes1 binding was generated by deleting the following nucleotides (underlined): Nbox2 – aC ACTAGc gc; Nbox3 – taG TGCTAg tg).

An RNA binding mutant was generated by changing two arginines that are essential for RNA binding region to alanines (Tat R52/53A).

The binding curve was generated by fitting the data simultaneously to one-site and two-site competitive binding models, and the F test provided a preferred model for the mean IC50 values (confidence interval 95%).

The mutant construct Luc-ADCY5-mut, mutation of the miR-17-5p binding site, was generated by PCR approach with primers musADCY5-SacI and musADCY5-MluI-mut (5′-CCACGCGTCAGAAGTTGCTTCTGAGTGTTAGTG-3′).

The plasmid containing site-directed mutations in putative p53 binding sites was generated by replacing the wild type Mlu I- Xho I fragment of pGL2-210 with a mutant fragment synthesized artificially pGL2-210BB, Table 1, Fig. 3).

The human CD133 promoter was cloned into the pG5 luciferase reporter vector, and the C/G to A/T mutation in the consensus p53-binding site was generated by site-direct mutagenesis.

Generation of putative binding modes: An initial binding mode for compound 1 was generated by manually docking the ligand into the ATP binding pocket of the TbGSK3 homology model with the requirement to establish hydrogen bonds to the hinge residues using Moloc.

Previously, the Her2-ECD binding Fcab clone H10-03-6 was generated by randomly mutating the structural loops (AB- and EF-loops) at the C-terminal tip of IgG1-Fc and subsequent selection for binding to the antigen [ 8].