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The apparent affinity constant (KD) of gRNA binding was extracted from data-point fitting using KaleidaGraph 3.5 and the following binding model: KD = [gRNAf][mRNAf]/[complex]. Where: complex = gRNA bound to mRNA; [gRNAfree] = [gRNAtotal]−[complex]; [mRNAfree] = [mRNAtotal]−[complex] [14].
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The list of experimentally determined RNA polymerase II binding sites of confluent HEK cells was extracted from the supplementary data of Sultan et al [ 16].
Insulin-like growth factor-I was extracted from IGF-binding proteins (IGFBPs) by using the acid-ethanol extraction method.
In order to evaluate the effectiveness of the docking protocol, co-crystallized ligand was extracted from the ligand binding domain of the protein and was re-docked into the same position.
Ovotransferrin was extracted from egg white using its metal (iron) binding properties.
Briefly, RNA was extracted from 200 μl aliquots of plasma using Trizol treatment and silica binding.
I was extracted from them".
DNA was extracted from adult S. japonicum.
RNA was extracted from sorted cell subsets.
Total RNA was extracted from cell lines.
Total RNA was extracted from 33 nodules.
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