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Because the Ligand1 molecule mediated the crystal packing in both the forms, the influence of crystal packing on the ligand binding was examined using a molecular dynamics (MD) simulation in aqueous conditions.
Peptide binding was examined using a Zeiss LSM 510 laser scanning confocal microscope (Carl Zeiss International, Germany).
The contribution of c-Jun binding to GR binding was examined using A-FOS, a dominant negative protein that heterodimerizes with c-Jun and prevents DNA binding [ 22, 33].
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The bindings were examined using 1H NMR, fluorescence and UV vis spectroscopic methods.
Estrogen receptor-binding ability was examined using an estrogen receptor (ER) competitive binding assay kit (Invitrogen, Carlsbad, CA) according to the manufacturer's instructions.
To ascertain that the nucleotides do bind the protein, fluorescent nucleotide binding to YqeH was examined using mant-GDP, mant-GDPNP.
Recently, the binding of Rho was examined using optical tweezers.
The binding of the inhibitors was examined using 1H-15N-HSQC experiments and differential chemical shifts were used to map the ligand binding sites.
The ssDNA binding activity of MutL was examined using a partial duplex DNA that consisted of 15 bp double-stranded DNA (dsDNA) with a 33-deoxythymidine nucleotide (dT33) 5'-overhang.
An example of the use of MS in biomarker discovery is given in which the albumin binding protein sub-proteome was examined using MALDI-TOF MS/MS.
The binding affinity of the lipopeptides for purified LPS was examined using our synthetic dansyl-polymyxin binding assay as previously reported.
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