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Annexin V binding was evaluated as a measure of cell cytotoxicity.
Binding was evaluated as the peptide's ability to displace the biotinylated control peptide (HA306 318) and was detected by a conventional ELISA.
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To assess the specific binding of the recombinant scFv apoptin to the CD22 surface antigen, the CD22+ Raji cells were exposed to the fusion protein and the binding was evaluated by FACS analysis as described before (Zarei et al. 2014).
The specificity of [ 18 F]flutemetamol binding was evaluated by adding 10 μM non-radioactive PIB (ABX, Radeberg, Germany) to the incubation solution, as previously described [9].
The polyamine-copolymer binding was evaluated by high-sensitivity differential scanning calorimetry.
DNA binding was evaluated by thermal denaturation and fluorescence measurements.
The relative amount of antibody binding was evaluated by ImageJ.
HERC2 and NCOA4 binding was evaluated by immunoblot.
Parameters of angiogenesis were evaluated as above.
The pentameric coiled-coil domain of cartilage oligomeric matrix protein (COMP) genetically fused to the Z domain of Staphylococcus aureus protein A, an immunoglobulin-binding domain (IBD), was evaluated as a viral antigen carrier complex.
We first assessed how rapidly TF binding differences accumulate among these five mouse genomes by determining the proportion of HNF4A, CEBPA, and FOXA1 TFBRs that reciprocally overlap between species in a qualitative manner; that is, how often TF binding in one species was evaluated as not identified in the homologous position in a second mouse species when comparing present-absent binding calls.
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