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Specific binding was established by coincubating with 10-, 25- and 50-fold excess of unlabelled oligonucleotide.
In this and subsequent experiments, specificity of binding was established by competition with free 20(S -OHC.
Streptavidin-coated donor beads were used in conjunction with glutathione-coated acceptor beads, and the binding was established by monitoring proximity-based luminescent signal.
The strength and mode of DNA binding was established by thermal melting stabilization, direct titration and viscometric studies of host duplex length.
For naïve T cells, subsequent firm binding was established by ICAM-1 and ICAM-2; however, for cancer cells, this binding could not be blocked by anti-ICAM-1 and ICAM-2 antibodies.
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Studies on the interaction between an archeal homolog of PCNA and FEN-1 (Flap-endonuclease 1) [33] reveal that the binding is established by the ordering of flexible C-terminal regions of PCNA and FEN-1 which creates an intermolecular β-sheet interface (although this was not observed in the human PCNA-FEN-1 complex [34]).
Local and remote bindings are established by creating new subscription relationships, supporting one-to-one, many-to-one, and one-to-many bindings (as specified in [13]).
The metal sensor binding mode was established by Job's plot and the combined fluorescence and 1H NMR spectroscopic method.
Tight ruthenium binding to serum albumin was established by joint use of spectroscopic and separation methods.
The Leu3p binding site of BAP2 was established by computer assisted analysis [ 41].
The time course of Annexin V binding to Jurkat cells was established by flow cytometry performed as described above.
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