Sentence examples for binding was done with from inspiring English sources

Blocking of nonspecific binding was done with BlockAce (Dainippon Pharmaceutical, Osaka, Japan) for 1 h at room temperature.

Blocking of nonspecific binding was done with equine normal serum diluted with bovine serum albumin (BSA)/tris buffered saline (TBS).

Membrane blocking to prevent nonspecific binding was done with TTBS buffer [10 mM Tris HCl (pH 7.6), 150 mM NaCl, 0.1 % Tween 20] containing 5%% skim milk powder.

Detection of primary antibody binding was done with donkey antirabbit horseradish peroxidase conjugated antibodies, followed by enhanced chemiluminescence detection (all from Amersham, Zurich, Switzerland).

Inhibition of endogenous peroxidase was done with H2O2 in methanol and blocking of nonspecific binding was done with caprine normal serum diluted with BSA/TBS.

Blocking of unspecific bindings was done with FCS/Tris 20%.

The protein stock solution contained either monomeric or hexameric protein and the binding was done at pH 7.4 with [GuHCl] < 10 mM.

Detection of Ki67 antibody binding was done using a Vectastain ABC kit with DAB chromogen (BD Biosciences Pharmingen).

To further determine the possibility of NF-κB binding to MMP-2/MMP-9 promoter binding sites, EMSA was done with specific oligonucleotides As shown in Figure  6C, the TGF-β1-stimulated NF-κB DNA binding activities were strongly inhibited by pinocembrin at the concentration of 5 μM.

In the absence of conditions for crystallizing full-length NEMO or larger fragments that contain the IKKβ binding domain, cocrystallizing compounds with only the IKKβ binding region itself, as was done with IKKβ701 745, would appear to be the best option.

Detection of primary antibody binding on western blot was done with BM Chemiluminescence Western Blotting Kit mouse/rabbit (Roche, Germany).