Sentence examples for binding was done at from inspiring English sources

Again, all centrifugations were done at room temperature, but the actual binding was done at 37°C.

The protein stock solution contained either monomeric or hexameric protein and the binding was done at pH 7.4 with [GuHCl] < 10 mM.

GST-SH2 precipitation assays to analyse pY protein binding were done at 4°C with total cell RIPA lysates. 1 mg of protein extract was mixed in each case with GSH beads loaded with either 50 μg GST or GST-SH2 fusion protein.

Blocking with 5% non-fat dried milk in TBS-T (50 mm Tris pH 8.0, 200 mM NaCl, 0.2% Tween-20) was performed for 1 hour, and primary antibody (p24 monoclonal, NIH AIDS Research and Reagent Program;183-H12-5C from Dr. Bruce Chesebro and Kathy Wehrly) binding was done overnight in the same solution at a dilution of 1∶1000.

Final screening for anti-V3 antibody binding reactivity was done at flask stage (OD>1.0).

The reported initial binding of the 'pioneer factors' was done at 48 hours after reprogramming induction whereas the ChIP-seq data used in our study was from ESCs that correspond to mature iPSCs.

Binding reactions were done at 4° C for 40 minutes in wash buffer.

Competition binding assays were done at 30 nM of [3H]-resveratrol in the presence of increasing concentration of cold resveratrol ranging from 0.1 nM to 100 µM.

A crab cake also needs binding, usually done with mayonnaise, eggs and a little bread crumb, as it is done at Costas.

All binding assays were done in duplicate.

All binding assays were done in triplicates.