Sentence examples for binding was done as from inspiring English sources

Quantification of mCherry_PGRP-SA binding was done as described above.

DNA binding was done as described in B & C, except loader was omitted in one sample.

Scatchard analysis of Rh-EGF binding was done as described previously [ 13, 14].

Detection and annotation of binding sites was done as described earlier (7).

Protein expression, purification, and retinol binding assays were done as for wild-type mSAA3.

Binding-kinetic curve-fitting procedures were done as described previously (Kuziemko et al., 1996; Wittenberg et al., 2012).

The comparative binding analysis was done by whole-cell ELISA as described in Materials and Methods.

The screening for cells expressing any mycobacterial-binding receptors was done by fluorescent microscopy as described in detail in [8] on a Zeiss inverted microscope Axiovert 40 CFL after incubation of the stably transfected NIH3T3 cells with M.bovis BCG expressing GFP prepared as described above.

To further determine the possibility of NF-κB binding to MMP-2/MMP-9 promoter binding sites, EMSA was done with specific oligonucleotides As shown in Figure  6C, the TGF-β1-stimulated NF-κB DNA binding activities were strongly inhibited by pinocembrin at the concentration of 5 μM.

In the absence of conditions for crystallizing full-length NEMO or larger fragments that contain the IKKβ binding domain, cocrystallizing compounds with only the IKKβ binding region itself, as was done with IKKβ701 745, would appear to be the best option.

Competition binding studies were done mainly as previously described [55].