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Nonspecific binding was determined with 5 μM -vesamicol.
Non-specific binding was determined with 1 μM unlabelled BN(1 14).
Non-specific binding was determined with 10 µM MLA.
Non-specific binding was determined with 20 µM unlabeled rosiglitazone.
Nonspecific binding was determined with 10 mM GBR 12935 (Sigma).
The biotinylated test antibody MAb 11A8 was added and its binding was determined with peroxidase conjugated streptavidin.
Similar(43)
Saturation constants (Kd and Bmax) for [3H]-AMPA binding were determined with the nonlinear curve-fitting program Ligand software (Biosoft, Cambridge, UK), using a range of unlabelled AMPA [75 10000 nM] added to 50 nM of [3H]-AMPA.
The effect of sequence fidelity on hybridization and aptamer binding were determined with microarrays synthesized with a light-exposure gradient.
Annexin V-binding was determined with the assay kit following the manufacturer's instructions.
Specific [3H]-AMPA binding was determined by subtracting nonspecific (determined with 50 µM of the AMPAR antagonist quisqualic acid) from total binding.
The relative cell binding was determined after staining with 0.1% crystal violet, solubilization with 10% acetic acid, and measurement of absorbance at 562 nm [ 18].
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