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Nonspecific binding was determined in parallel experiments utilizing 7A (5 μM) as a competitor.
Nonspecific binding was determined in the presence of 10-μM -pentazocine.
Nonspecific binding was determined in the presence of 7A (5 μM) as a competitor.
Nonspecific binding was determined in the presence of 10-μM DTG and 1-μM -pentazocine.
In both assays, non-specific binding was determined in the presence of haloperidol (10 μmol/L).
Unspecific binding was determined in the presence of a high concentration (0.5 100 μM) of the non-labelled competitor substance.
Similar(12)
Nonspecific binding is determined in the presence of 10 μM triamcinolone.
Non-specific binding was determined as binding in the presence of 10 mM non-radioactive ouabain.
Assessment of peptide binding was determined with sensorgrams, in which the arbitrary response units (RU) were plotted as a function of time.
Chromatin was immunoprecipitated overnight with anti-RNA polymerase II (10 µg), anti-TFIID (10 µg), or anti-acetylated histone H3 (5 µg) or H4 (4 µg) antibodies; the specificity of binding was determined using controls in which the primary antibody was omitted.
Non-specific binding was determined by binding in the presence of 50-fold molar excess of cold ligand.
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