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Nonspecific binding was determined from samples that contained 10 μM of cold haloperidol.
Background binding was determined from the absorbance generated in wells with blocking solution alone.
Non-specific binding was determined from fluorescence values of all non-specific probes.
Start of equilibrium binding was determined from a plot of number of particles vs frame.
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The enthalpy change associated with each injection of ligand was plotted versus the [CTN]/[HSA] molar ratio (Figure 2, lower panel), and the ΔH, Ka, the free energy change (ΔG) associated with binding were determined from the plots.
From dimensional measurements, the critical contact angles for binding were determined for all products and ranged from 3° to 5°.
In all experiments, nonspecific binding was determined and subtracted from total binding to obtain specific binding.
Non-specific binding was determined by incubating slices from a vehicle-treated animal in assay buffer that contained the appropriate radioligand and a high concentration of a non-radioactive competitor for the target.
A 1.83 ± 0.10% CD-31 binding was determined in the tumor tissue from the control mice, whereas the samples from sunitinib-treated animals showed reduced binding of 0.58 ± 0.05% CD-31 (n = 8/4 - tumors from four mice; p < 0.001).
Specific binding was determined by subtracting nonspecific binding from total binding and free ligand concentration was estimated by subtracting total bound ligand from added ligand.
Specific binding was determined by subtracting the background absorbance from the absorbance in experimental wells.
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