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The nonspecific binding was determined at the presence of 50 μL of unlabeled ligand.
In the first set of experiments, NP-9 and NP-29 cells were infected with Adhcellsor Adctrol at different MOIs, and annexinV binding weredeterminfecteddifferent times post-infection.
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The level of non-specific binding was determined by adding unlabelled GTPγS at a final concentration of 40 µM.
Nonspecific binding was determined with 5 μM -vesamicol.
Peptide binding was determined using ELISA.
Specific binding was determined by subtracting values of non-specific binding from that of total binding.
Non-specific binding was determined with 10 µM MLA.
Non-specific binding was determined with 20 µM unlabeled rosiglitazone.
Nonspecific binding was determined using 1 µM indatraline.
Nonspecific binding was determined with 10 mM GBR 12935 (Sigma).
In all experiments, nonspecific binding was determined and subtracted from total binding to obtain specific binding.
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