Sentence examples for binding was determined as from inspiring English sources

Non-specific binding was determined as binding in the presence of 10 mM non-radioactive ouabain.

Specific [3H]-DEX binding was determined as the difference between total and nonspecific binding and was expressed as sites/cell.

Specific binding was determined as the difference between the polarization (mP) reading in the absence of competitor to the reading in the presence of a 1000-fold excess of unlabeled reporter peptide.

Serum IgG binding was determined as described above using alkaline phophatase-labeled anti-human IgG antibodies and 4-methylubelliferyl phosphate (1 µg/mL in 0.2 M TRIS, pH 9.5) (Sigma, St . Louis.

Saturable ethylene binding was determined as described by incubation of samples in sealed glass chambers containing either 14C-ethylene (0.1 µL/L) or 14C-ethylene (0.1 µL/L) plus 12C-ethylene (100 µL/L) [10], [22].

Nonspecific binding was determined as the amount of H-NMS bound in the presence of excess/1  μ M dexetimide.

Using the same method as described above to obtain the mean values and the standard errors of the mean from three repeated experiments for Δ H°, Ka, n, − TΔ S°, and Δ G° for CaM Fas DD WT binding, the thermodynamic parameters for CaM Fas DD V254N binding were determined as shown in Table 1.

Antibody binding was determined using ECL as described above.

The concentration of competitor peptide inducing 50% inhibition of marker peptide binding was determined, and expressed as the 50% inhibitory concentration (IC50 in μM).

Cell-bound activity was determined and expressed in % of incubated activity, specific binding, after subtraction of nonspecific binding, was determined and expressed as percent of incubated activity and immunoractivity determined using double inverse plot (Lindmo et al, 1984) with extrapolation at infinite antigen excess.

Plasma protein binding was determined using equilibrium dialysis as previously described.