Specific binding was detected with the Dako REAL EnVision detection system (peroxidase/DAB+, rabbit/mouse) and visualized with diaminobenzidine.
Antibody binding was detected with the enhanced chemiluminescence (ECL) detection system (Amersham Biosciences, Piscataway, NJ).
Antibody binding was detected with a horseradish peroxidase (HRP -coupled secondary antibody followed by cHRP -coupledence detection (ECL Plusecondaryantibodyacia, Uppsala, Sweden).
Antibody binding was detected with EnVisionTM Dual Link System-HRP DAB kit (K4010, Dako).
No binding was detected with the scrambled oligo (Fig. 3b, top left panel, Supplementary Fig. 5).
In the case of immobilized antibodies anti-Cry1Ac or anti-Cyt1Aa the binding was detected with an antibody anti-RGS-His-HRP.
The binding was detected with an anti-His mAb.
The binding was detected with HRP-conjugated secondantibodybody.
In contrast, no binding was detected with the antisense probe.
No binding was detected with GST alone (Fig. 6Biv, lane 5).
The binding was detected with an ALP-anti-His tag mAb [14].
