Sentence examples for binding was detected when from inspiring English sources

There was no binding of Stx2 to platelet-bound neutrophils and no binding was detected when the primary anti-Stx2 antibody was omitted.

Also, no significant binding was detected when MUC1- Lovo cells were used (not shown).

In contrast, no antibody binding was detected when the oocyst extract was reduced (Table 2; Figure 3a).

Previous study showed that no NRF2ΔN/KEAP1 binding was detected when NRF2ΔN was co-expressed with KEAP1 [ 16].

While C-terminally tagged QIL1 efficiently co-purified with endogenous MIC60, MIC19, MIC27, and MIC10 when mitochondria were solubilized with 1% digitonin, no specific binding was detected when mitochondria were solubilized with Triton X-100, consistent with an association of QIL1 with the mature MICOS complex.

In cellulase, CBH is found to have a higher affinity to lignins than EG. Lower lignin-binding was detected when using the enzyme without CBM and the linker domain, thus, CBM was found to play an important role in protein adsorption [ 2], which could explain the least inhibition of BG by lignin.

The allosteric binding site was detected when trying to dock the inhibitor onto the kinase using a rigid geometric protein−protein docking procedure, without any distance constraints.

The results showed that high Pi markedly promoted the binding of Klf5 to proximal region of the Runx2 promoter (-185 bp–27 bp), which contains Klf5-binding sites 1 4;no significant binding of Klf5 was detected when distal Runx2 promoter region containing Klf5-binding sites 5 8 was amplified.

Moreover, no c-di-GMP binding to MlrA was detected, when a csgD promoter fragment containing the MlrA binding site (Ogasawara et al, 2010b) was added or when we used the His6-tagged YdaM-GGDEF domain alone (data not shown; this construct does not contain the PAS domain that inhibits the interaction of YdaM-GGDEF with MlrA-CTD; see Figure 3).

The binding of the complement inhibitors was also confirmed using purified proteins and washed activated platelets (data not shown); however, no binding of C1INH was detected when washed, TRAP-activated platelets and purified protein were used.

Also, no unspecific binding to the antibody was detected when cellular extracts from wild type 293T were used in this assay (data not shown).