The specific binding was detected using the chemioluminiscence detection kit ECL (Amersham Bioscience) following the manufacturer indications.
Secondary antibodies conjugated to horseradish peroxidase (Amersham Bioscience, UK) and specific antibody binding was detected using the chemiluminescence detection reagent ECL Plus (Amersham BioScience, UK).
Antibody binding was detected using enhanced chemiluminescence detection system (GE Healthcare).
Antibody binding was detected using Amersham ECL detection reagents (GE Healthcare, cat. no. RPN2209).
Specific antibody binding was detected using a chemiluminescence detection system (Pierce, Rockford, IL, United States), according to the manufacturer's recommendations.
Antibody binding was detected using the Ultravision LP detection System (Thermo Fisher Scientific, Fremont, CA, USA) and Bright DAB (Medac, Wedel, Germany).
Antibody binding was detected using an enhanced chemiluminescence detection system (Cell Signaling Technology, Milano, Italy).
Secondary-antibody binding was detected using an enhanced chemiluminescence detection kit (Thermo Fisher Scientific, USA) according to the manufacturer's instructions.
Secondary-antibody binding was detected using an enhanced chemiluminescence detection kit (Amersham, Piscataway, NJ, USA) according to the manufacturer′s instructions.
Membranes were rewashed extensively and binding was detected using Enhanced Chemiluminescense western blotting detection system (Amersham Pharmacia Biotech, Piscataway, NJ, USA), according to the manufacturer's instructions.
Antibody binding was detected using an enhanced chemiluminescence HRP substrate detection kit (Millipore, Watford, UK).
