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Fluorescence activated cell sorting (FACS) analysis showed that FD10 bound with cells harboring pcDNA3.1-FXYD6 (Fig. 1D, left panel), whereas no such binding was detected in cells with pcDNA3.1-empty vector only (Fig. 1D, right panel), demonstrating that FD10 could recognize the FXYD6 with native conformation.
Stx2 binding was also examined on purified platelets, monocytes and neutrophils and binding was detected in a similar manner.
Residual Ubp2-Rsp5 binding was detected in the absence of Rup1, implying that Ubp2 and Rsp5 can interact, either directly, or possibly through another unknown adaptor protein.
The binding was detected in Western-blot with goat anti-human IgG (Fab specific -ALP and goat anti-human IgG (Fc specific -ALP (SigmandCA, USA) respectively.
Cell surface fiber binding was detected in a great majority of the cells, irrespective of the level of GFP or hexon expression (Figures 4, upper right panel and Figure S3).
Rosetting and CD36 binding began to decline already after 25 generations of growth and had disappeared at 80 100 generations in 3D7S8.4, and no CSA binding was detected in this parasite (Figure 2).
Robo1 (A ), Robo2 (B ), and Robo2∆Ig2 (D ) bind efficiently to Slit, while little to no binding is detected in cells expressing Robo2∆Ig1 (C ) or Robo2∆Ig1+2 (E ).
High intensities of cell surface fiber binding were detected in A549 cells following CD46-binding species B Ad11 and Ad35 infection (Figure 5D).
Elba factor binding is detected in 0 6 hr nuclear extracts, but it is absent in 6 12 hr (and 6 18 hr) nuclear extracts (Aoki et al., 2008 ).
(B ) Signal tracks showing Zfp335 occupancy at three target genes (Rbbp5, Polr2e, Pes1 ) for which no reduction in binding is detected in blt / blt thymocytes.
