Specific antibody binding was detected by chemiluminescence detection.
Antibody binding was detected by the ECL detection kit (Amersham, Piscatawya, NJ, USA), followed by autoradiography (Kodak X-Omatic AR).
Antibody binding was detected by enhanced enhanced ECL detection kit (UK Amersham International plc).
Antibody binding was detected by enhanced chemiluminescence (ECL) detection kit (Thermo Fisher Scientific, Waltham, MA, USA) and captured on X-ray film.
Antibody binding was detected by means of the UltraVision LP detection system according to the manufacturer's recommendations (Lab Vision Corporation).
Antibody binding was detected by autoradiography using the enhanced chemiluminescence immunoblotting detection system (Amersham Biosciences) and quantified by densitometry using Image J software (NIH).
For IHC, antibody binding was detected by using the "Envision+" System-HRP (DAB) followed by chromogen detection with diaminobenzidine (DAKO).
The binding was detected by Streptavidin-HRP conjugate.
The binding was detected by DAB solution (ZSGB-BIO).
Both proteins were expressed in HEK 293T cells, and binding was detected by measuring the absorbance of the nitrocefin substrate.
Soluble PAB proteins purified as in vivo biotinylated HisTag-PAB-TolA-AviTag fusion products were added to cells and the binding was detected by streptavidin-PE conjugate.
