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In receptor-binding studies, ZD4054 specifically bound to ETA with high affinity; no binding was detected at ETB.
In contrast, E-cad- 3A -GFP, which was dE-cad- 3A -GFPmb E-cad- 3A -GFPE-cad- 3A -GFP apical region of the cell–cell junction.
Thus Twist1 binding was detected at genomic sequences associated with Wnt pathway genes and Wnt interacting genes in developing ECCs and limb buds.
Significant NICD binding was detected at HLHmβ, but not at HLHm3 when the Nipped-B subunit of the kollerin complex that loads cohesin onto chromosomes was depleted.
Su(H) binding was detected at these sites in untreated cells, and the levels were not significantly altered by cohesin, kollerin, or PRC1 depletion.
Thus, Twist1 binding was detected at small group of shared binding regions in ECC, limb bud, and PNST cells, which may represent common targets of Twist1 in multiple cell types.
Similar(48)
No Rpol II binding is detected at either of the low gene-density regions, while peaks of binding are found scattered throughout the other three regions.
STAT1 and RXRA binding were detected at the respective ranks of 10 and 12 (Bonferroni corrected P-values: q = 0.0009 and q = 0.0018, respectively).
Indeed, we find that Esrrb binding is detected at ∼70% of genes (8,149 out of 12,051) that show significant expression by RNA-seq (>0.5 RPKM) in ground state ESCs (Marks et al., 2012).
Inspection of our ChIP data suggest that the regulatory effect of Oct4 might be direct because Oct4 binding is detected at an enhancer region close to the Otx2 locus, and this region shows evidence of increased H3K27 acetylation as the Otx2 locus becomes activated.
Following incubation for 1 h with a secondary horseradish peroxidase-conjugated antibody (1 1000 dilution), specific binding was detected by colorimetric estimation at 450 nm using either mutant DNA or no protein addition controlled for nonspecific binding.
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binding was attained at
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