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When cell-derived membranes were coated on the quartz nanosensor, however, significantly more binding was detected, and the QCM-derived kinetic measurements suggested the existence of an interacting receptor in the target membranes.
Antibody binding was detected and visualised using horseradish peroxidase Vectastain ABC Elite kit (Vector Laboratories, Peterborough, UK) followed by a 3,3'- diaminobenzidine/nickel staining kit (Vector Laboratories).
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Lar-AP-containing cell supernatants were incubated with live-dissected late stage 16 Drosophila embryos, and binding was detected with secondary and tertiary antibodies.
Specific binding was detected between SOLD1 and the type I collagen (COL1) coated plate (Fig. 5A).
Hsp70 binding was detected for CHIP and TPR2, as reported previously [34], [57].
No specific binding was detected between SOLD1 and any other ECM.
While dasatinib binding to wild type Abl clearly influenced Abl conformation, no binding was detected between dasatinib and T315I.
The qChIP experiment showed that p73 binding was detected in untreated and HU-treated cells.
I-rHuEpo or biotinylated-rHuEpo binding was detected on erythroid and some myeloid-cell types with a dissociation constant (Kd) of ∼100 pM [ 29, 53, 54].
Three concentrations of ricin (0.1, 1.0, and 10.0 pg/ml) were tested, and binding was detected using biotinylated goat anti-ricin Ab and HRP-streptavidin (HRP-SA) for ECD.
A very weak binding affinity was detected for the structure formed by oligomer d, whereas no binding was detected for oligomer e and two controls, single stranded and double stranded DNA.
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