Sentence examples for binding was detected after from inspiring English sources
Antibody binding was detected after incubation with appropriate secondary antibodies conjugated with HRP, with the membrane-bound antibodies visualized by luminal chemiluminescence ChemiDoc XRS (Bio-Rad, Hercules, CA, USA).
Antibody binding was detected after 2 h incubation with a 1 2500 IgG or 1 5000 IgM of rabbit anti-mouse immunoglobulin conjugated to peroxidase (Kirkegaard & Perry Laboratories, Inc., Gaithersburg, MD).
Promoter regions of SOK1, HYR1 and ECE1 genes are known to bind the Efg1 regulator relatively late during hyphal induction [ 26] or during biofilm formation [ 25], while no Efg1 binding was detected shortly (30 min) after hyphal induction [ 13].
Plates were coated with TT, incubated with plasma samples and after washing, binding was detected using horseradish peroxidase (HRP -conjugated anti-monkey IgG antibodies.
After isolation of PBMCs and in vitro activation of T-cells, a dose dependent enhancement of biotinylated soluble ICAM-1 binding was detected in presence of compound 1 after staining with streptavidin-PE.
Antibody binding was detected with substrate A and B. After the reaction was stopped with H2SO4, the absorbance at 450 nm was measured by an ELISA reader (Bio-Rad 680, USA).
Then, AP-RR36 in situ binding was detected using AP fluorescent substrates or, after careful fixation of free-floating slices, using anti-AP antibodies in combination with cell markers.
After washing, antibody binding was detected with a phycoerythrin-conjugated IgG-F ab 2 IgG-F ab 2Dianova).
After washing, antibody binding was detected using a Vectastain ABC Elite peroxidase system (Vector laboratories Inc).
After washing, antibody binding was detected by adding 100 μl poly-nitrophenylphosphate diluted in Tris buffer (Sigma Chemicals, Zwijndrecht, The Netherlands).
After washing, antibody binding was detected using diaminobenzidine substrate-chromogen (Sigma-Aldrich, Zwijndrecth, the Netherlands) for 5 min at room temperature.
