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No binding was detectable to GST control protein.
Significant Mysm1 binding was detectable to a fragment −1-k upstream of the transcriptional start site (primer set 20) and to a fragment around the transcriptional start site of exon 1B (primer set 34) in activated thymocytes.
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Albeit high-affinity NSC48300 binding sites in both the active and inactive Taspase1 structure were identified, no binding was detectable at or close to the catalytic nucleophile, Thr.
Under repressing conditions Pho2 occupied its binding site close to UASp1, but no binding was detectable at N-2 sequences (Fig. 5).
Interestingly, when we analyzed the binding of ZO-2 protein in pull-down and co-immunoprecipitation experiments, association of ZO-2 wasignificantlyly attenuated in the phospho-mimetic Occ-T400E/T404E/S408E-transfected cells, whereas in Occ-T400A/T404A/S408A-transfected cells a minor but not significant increase in ZO-2 binding was detectable compared to wildtype occludin.
The addition of the BODIPY-FL fluorophore resulted in molecules with much reduced potency, which was probably due to a reduction in affinity, as no binding was detectable.
With the exception of the BN3 derivative for which no specific binding was detectable, all compounds showed saturable binding with K d in the order of 10−9 M and B max values in the order of 105 receptor sites per cell.
LactC2 binding exhibited a lag period of 1 2 min before binding was detectable.
In control experiments where FLAG-Occ or CK2 were transfected alone, no binding was detectable.
Apramycin binding was detectable, using spectroscopy at low nM concentrations and was visibly red by ∼62.5 nM.
Ago2 binding was detectable in hypoglycemic conditions on UTR-S1 mRNA, but not on UTRm-S1 mRNA.
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