Their ability to bind insoluble cellulose was demonstrated using a cellulose-binding assay involving the micro-crystalline cellulose, Avicel.
The binding specificity of 9a was demonstrated using several representative sugar phosphate phosphatases from the HAD enzyme superfamily, the T6PP protein fold family of origin.
Cellulose-binding ability of Xyl10A CBM1 was demonstrated using quartz crystal microbalance with dissipation monitoring.
In both cases, a clear and ligand-specific binding of the biotinylated molecules was demonstrated using streptavidin coupled to Quantum Dots, which provided a stronger signal compared to streptavidin conjugated with traditional fluorophores (not shown).
As shown in Figure 2A, the specific binding of the DNA probe to STAT3 was demonstrated using supershift and competition analyses.
Although the TCTP receptor has not been identified, binding between TCTP and TCTP receptor has been demonstrated using FACS analysis in several reports [29], [30].
Direct binding to class I and class IIa HDACs is demonstrated using co-immunoprecipitation assays and binding of PLU-1/JARID1B to in vitro translated HDACs.
Furthermore, direct interaction between delphinidin and ERα activator site is demonstrated using both binding assay and docking.
Collectively, the direct binding of KU174 to recombinant Hsp90 is demonstrated using DARTS, and SPR experiments as well as biotinylated KU174 that co-immunoprecipitates Hsp90 from tumor cell lysate, which can be eluted in an ATP-dependent manner.
