Sentence examples for binding was defined with from inspiring English sources

Non-specific binding was defined with 10 µM of 5-HT in 5-HT1AR and 5-HT7R binding experiments, whereas 10 µM of methiothepine or 10 µM of haloperidol were used in 5-HT6R anD2L2L assays, respectively.

DA transporter (DAT) binding was defined with 50pM [125I]RTI-55 (Perkin Elmer) in the presence of 1 µM fluoxetine (Sigma) in buffer A (Tris 50 mM, pH 7.4 ; NaCl 120 mM ; KCl 5 mM ; BSA 0.25%).

Specific binding was defined with 40μM benztropine (Sigma, St . LouisMO), and amounted to 95% of total binding.

Briefly, uptake and binding assays used a high sodium, low potassium buffer containing glucose and tropolone, and the non-specific binding was defined with 1 μM CFT as in our previous work.

Nonspecific binding was defined in adjacent sections by co-incubation with 50 μM of unlabeled β-mercapto-β,β-cyclopentamethylenepropionyl 1, O-me-Tyr2, Arg8]-vasopressin (Perkin Elmer) or 50 μM of the selective oxytocin ligand [Thr4,Gly7] oxytocin (Perkin Elmer).

AT1R specific binding was defined as [I]-[SarIle] Ang II binding after tissue incubation with the AT2R antagonist PD-123,319 and in actual fact included AT1R binding as well as non-AT1R/non-AT2R binding.

Specific binding was defined as total minus nonspecific binding.

Non-specific binding was defined by 1 µM NTX.

Nonspecific binding was defined as binding in the presence of 100 µM phentolamine hydrochloride.

Non-specific binding was defined by unlabelled ZM241385 (1 μM).

Nonspecific binding was defined by unlabeled 100 μM β-NAD+.