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Nonspecific binding was defined using 1 μmol/L [Pyr]apelin-13.
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Nonspecific binding in saturation binding assays was defined using membranes from nontransfected HEK 293 cells.
The binding site was defined using the αC atoms of conserved aromatics Phe206, Tyr254, and Tyr109, with a binding site radius of 10 Å.
Non-specific binding (NSB) was defined using 100 nmol/L of CCL4.
For docking studies the binding site was defined, using LeadIT [ 36] with the aid of reference ligand which had cocrystallized with the enzyme.
The active site was defined using AutoGrid.
While the optimal mode I and II 14-3-3-binding motifs were defined using phosphopeptides [ 18, 19], target sites in proteins must satisfy the specificity requirements for both 14-3-3s 14-3-3s 14-3-3s kinands thet create the sites in the first protein
The ligand is subsequently docked into the protein binding site (using settings that can be defined using the API during model calibration), and maximally 50 poses with mutual RMSD of 2.0 Å are retained for each of the six rotated configurations.
These schemas are defined using XML.
Classes are defined using Java syntax.
Non-specific binding was defined with 10 µM of 5-HT in 5-HT1AR and 5-HT7R binding experiments, whereas 10 µM of methiothepine or 10 µM of haloperidol were used in 5-HT6R anD2L2L assays, respectively.
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