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The threshold of low signal, corresponding to MPIO binding, was defined to be 3 standard deviations below the mean signal intensity of the precontrast root on signal intensity histograms.
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The binding pocket was defined to include amino acid residues within a 4-Å reach around Site-2.
The binding pocket was defined to consist of residues A3, A15, G16, and A21, which locates roughly at the top of the 5′-terminal of the G-quadruplex.
Relevance of peptide-HSP72 interaction analysis to RNP-HSP72 binding was defined by examining the ability of free peptide to inhibit RNP-HSP72 interaction on BIAcore sensors.
Consistency in Calcofluor binding was defined as strains that always were able to bind Calcofluor when tested repeatedly on different or same type of surface.
Nonspecific binding was defined from the autoradiograms of tritiated labelled ligand binding to white matter in human AD brain slices.
Specific binding was defined with 40μM benztropine (Sigma, St . LouisMO), and amounted to 95% of total binding.
Nonspecific binding was defined by heterologous displacement of 1 μM LY480084 (mGlu1 antagonist) in select wells.
Non-specific binding was defined with 10 µM of 5-HT in 5-HT1AR and 5-HT7R binding experiments, whereas 10 µM of methiothepine or 10 µM of haloperidol were used in 5-HT6R anD2L2L assays, respectively.
Specific binding was defined as total minus nonspecific binding.
Non-specific binding was defined by 1 µM NTX.
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