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Nonspecific binding was defined from the autoradiograms of tritiated labelled ligand binding to white matter in human AD brain slices.
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Specific binding was defined as total minus nonspecific binding.
Non-specific binding was defined by 1 µM NTX.
Nonspecific binding was defined as binding in the presence of 100 µM phentolamine hydrochloride.
Non-specific binding was defined as binding in the presence of 10 µM GTPγS, and basal binding indicates binding in the absence of agonist.
Non-specific binding was defined by unlabelled ZM241385 (1 μM).
Nonspecific binding was defined by unlabeled 100 μM β-NAD+.
Here, the contribution from nonspecific binding is defined as a linear relation between analyte concentration and amount bound.
A scoring matrix was defined from the 48 experimentally determined E. coli FUR binding sites [ 34] using the Multiple Em for Motif Elicitation (MEME) tool.
The aligned tRNAGln sequences are given in Additional file 7. The N-terminal catalytic domain and the C-terminal anticodon-binding domains of GluRS were defined from multiple aligned GluRS sequences by annotating residues corresponding to 1-322 and 323-468 of T. thermophilus GluRS as the N- and C-terminal domains, respectively [ 10].
Nonspecific binding in saturation binding assays was defined using membranes from nontransfected HEK 293 cells.
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