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Nonspecific binding was defined by heterologous displacement of 1 μM LY480084 (mGlu1 antagonist) in select wells.
Non-specific binding was defined by 1 µM NTX.
Nonspecific binding was defined by unlabeled 100 μM β-NAD+.
Non-specific binding was defined by unlabelled ZM241385 (1 μM).
Non-specific binding was defined by addition of 10 μM IP3.
Antigen-negative background binding was defined by the fluorescent intensity of isotype controls.
Similar(51)
Preliminary studies in our laboratory have shown that N peptides representing the carboxyl terminal 15 amino acids of CDV and MV bind with comparable affinity, supporting the idea that binding is defined by a greater degree of primary sequence than was originally envisioned.
These data suggest that the determinants required for CFH binding are defined by conformation rather than contiguous linear elements.
Within ChiP-seq data sets, the statistics of specific and non-specific DNA-protein binding is defined by a mixture of sample size-dependent skewed functions described by Kolmogorov-Waring (K-W) function (Kuznetsov, 2003) and exponential function, respectively.
The recognition capability of MoRFs and their ability to undergo induced folding at binding are defined by their specific amino acid biases, since these regions usually have a much higher content of aliphatic and aromatic amino acids than disordered regions in general and therefore are computationally identifiable.
Nonspecific binding was defined in adjacent sections by co-incubation with 50 μM of unlabeled β-mercapto-β,β-cyclopentamethylenepropionyl 1, O-me-Tyr2, Arg8]-vasopressin (Perkin Elmer) or 50 μM of the selective oxytocin ligand [Thr4,Gly7] oxytocin (Perkin Elmer).
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