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Nonspecific binding was covered using 5% bovine serum albumin (BSA).
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Non-specific bindings were covered using 5% bovine serum albumin (BSA), Sections were then incubated using H2O2 (3%) for 30 minutes to block endogenous peroxidase activity and washed in PBST and distilled water.
When the process was finished, slides were covered using a fluorescent mount medium and cover-slips.
Then, 370-bp from the upstream region of ptk was used as the promoter P ptk to make sure that the binding site of AraR was covered [ 47].
Devices were then imaged using an Andor iXON 885 fluorescent camera, and at least 10 images were analyzed for binding to channel surface by calculating total surface area covered using ImageJ.
Peptide binding was determined using ELISA.
Nucleotide binding was measured using a standard filter-binding assay.
Gold titles were engraved in the chocolate-colored binding and the pictures were covered with waxy paper.
The binding of any genomic region that is covered by a probe is represented by the log2 binding ratio of the probe; the binding of a region that is not covered by a probe is zero.
That is, data collected using the short-chambered design, where half of the shunt's ventricular catheter holes were covered by the gel, had significantly less total shunt-cell binding per square millimeter than data collected using the long-chamber design, where none of the shunt's ventricular catheter holes were initially covered by the gel (P < 0.001).
Two different properties: steric, electrostatic, assumed to cover the major contributions to ligand binding, were used to generate the 3D-QSAR model.
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